Olympus fluoview FV1000 confocal laser scanning microscope
CAN’s dedicated in-house laser scanning confocal microscope is used for high-resolution imaging of both fixed and live fluorescent specimens. The microscope is currently configured with 3 fluorescence channels and 3 lasers. It can perform high-speed imaging (up to 8frames/sec) using a fully motorized scan unit in the XY and Z plane. Recently, we have added a stage top incubation system including temperature, CO2 and humidity control to allow for long-term live cell imaging.
The confocal microscope is one of the most frequently used resources at CAN. Specific applications currently include: immunohistochemistry of brain slices from multiple animal models; and immunocytochemistry of endogenous proteins in immortalized and primary cell lines. Using the confocal microscope, we investigate the cellular localization and interactions of proteins of interest. We also look at exogenous proteins tagged to fluorescent reporters and assess their function in live and fixed cells.