ANTEC Neurotransmitter Analyzer UHPLC
The Ultra High-Performance Liquid Chromatography (UHPLC) system allows separation and quantification of small molecules in biological fluids. At CAN, we use this to measure the concentration of different neurotransmitters in specific brain areas. The system is able to analyze monoamines (dopamine, serotonin, noradrenaline and their metabolites) all from the same sample with a 7-minute run. Moreover, amino acids (GABA and glutamate) can also be measured in just 15 minutes. The efficiency of the analysis is guaranteed by an autosampler able to accommodate up to 90 samples, keep them refrigerated and analyze them continuously.
Along with microdialysis technique, we are able to assess the activity of specific neuronal populations in discrete brain areas through the measurement of the extracellular concentrations of these neurotransmitters. This approach is applicable to any rodent model in the field of neuroscience. In the study of neurodegenerative diseases, this is of particular relevance to understand what changes in the brain upon toxic insults or genetic modifications. With these milestones in mind, we are undertaking the investigation of the circuitry of a subset of brain regions (i.e. the basal ganglia) in genetic PD models, to assess the impact of these manipulations on neuronal activity and connections.