Applied Biosystems 3730xl DNA Analyzer, 96 Capillary
We use the Applied Biosystems 3730xl DNA Analyzer for high-throughput genetic analysis. The system offers reliable sanger sequencing of up to 900 base pairs and genotyping at an affordable cost per sample. Unlike other sequencing methods, sanger sequencing using the 3730xl can be completed within a day and does not require much operator input. Additionally, the 3730xl is ideal for Amplified Fragment Length Polymorphism (AFLP), Single Nucleotide Polymorphism (SNP) analysis, mutation detection, microsatellites and fragment analysis.
We utilize our 3730xl sequencer widely with all of our projects. Sanger sequencing is considered the most accurate way to confirm SNP detection and can provide accurate and easily reproducible data.Currently, we use our 3730xl to confirm SNP detections made by our 5500xl SOLiD™ next-generation sequencer, exome sequencing, and validation of positives from routine gene screening via Sequenom® SNP genotyping platform and TaqMan probes.